N-(p-amylcinnamoyl) Anthranilic Acid (ACA) is a broad spectrum Phospholipase A2 (PLA2) inhibitor and TRP channel blocker.

N-(p-amylcinnamoyl) Anthranilic Acid通过直接作用调节不同的TRP通道。因其在TRPM2上的高效能和高效率，N-(p-amylcinnamoyl) Anthranilic Acid能够与其他阻断剂结合使用，作为研究H2O2诱导的Ca2+信号传导和TRPM2通道在原生细胞中的生物学功能的药理学工具[1]。N-(p-amylcinnamoyl) Anthranilic Acid同时也是磷脂酶A2的抑制剂，在50 μM的浓度下阻断花生四烯酸的释放[2]。

Measurements of [Ca2+]i were carried out on TRPM8-transfected HEK293 cells grown in 96-well microplates. Cells were loaded with 1?μm fluo-4 for 30?min at 37°C in a standard solution. Subsequently, the standard solution was exchanged for a solution containing 1?mm EGTA instead of 2?mm CaCl2, and plates were inserted into a fluorometric-imaging plate reader. The fluorescence was excited at 485?nm, emitted at 535?nm and corrected for the background fluorescence. Fluorescence values were measured after addition of ACA, menthol and 3?mm CaCl2 to the EGTA-containing solution. ACA, menthol and 3?mm CaCl2 were applied consecutively in the given order with a time delay of 2?min between each application[1].